Available in tube and microplate formats, these reducing agent compatible rac bca kits extend the already broad reagent compatibility of the bca protein assay protocol to include. Determine acceptable wavelengths for measuring protein assays. Jun 19, 2018 the principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration. Bca protein assay kit ab102536 provides a simple, rapid, detergent tolerant up to 5% procedure for determining the concentration of proteins in solution. Mixing together the three micro bca reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. Eliminate interfering substances from samples for bca protein assays. Some substances, including most reducing agents, interfere at even low concentrations in the bca. Western blot on a10 protein isolates protein samples 1525 g were separated using 10% enac, 4%20% gradient nf b or. Materials solutions bca assay pierce cat 23225 for 1 l kit. When samples do not contain substances that interfere with the specific reagent or dye. Aug 02, 2010 the total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric.
Thermo scientific pierce bca protein assay kit bca protein. Number description 23225 bca protein assay kit, sufficient reagents for 500 test tube or 5,000 microplate assays 23227 bca protein assay kit, sufficient reagents for 250 test tube or 2,500 microplate assays kit contents. Elena deryugina measurement of protein concentration is important in many biological applications, e. Bicinchoninic acid protein assay kit bca1 technical bulletin. Interleukin17 reduces enac via mapk signaling in vascular. Pierce bca protein assay kit university of arizona. Pierce bca bicinchoninic acid reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The micro bca protein assay kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature 60c, test tube procedure only. Bca protein assay reagent a is a component of the pierce bca protein assay kit, a twocomponent, highprecision, detergentcompatible assay that is used for total protein concentration determination compared to a protein standard. Streamline bcabased protein quantitation molecular devices. Additional information and online ordering used in more labs than any other detergentcompatible protein assay, pierce bca reagents provide accurate determination of protein concentration with most sample types. The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. Measuring protein concentration using the bca protein assay. Measuring protein concentration using the bca protein assay with.
This is the protocol to be used in the determination of total protein in brain tissue by the pierce bca method. Measuring protein concentration using the bca protein. Eliminate interfering substances from introduction. The bicinchoninic acid assay bca assay, also known as the smith assay, after its inventor, paul k. We use the pierce micro bca kit to determine protein concentrations in solutions that contain 0. The bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. The amount of purplecolored product formed during the reaction can be correlated to the amount of protein in the bca protein assay. We recommend the use of gloves, lab coats and eye protection. Thermo scientific pierce rapid gold bca protein assay kit is twocomponent, highprecision, detergentcompatible assay optimized to measure at 480nm total protein concentration compared to protein standard curve of known concentrations. If you are not satisfied with the performance of a pierce product, please contact pierce or your local distributor. Accurately measure the total protein concentration in your sample with this twocomponent, highprecision, detergentcompatible assay reagent set. Thermo scientific, following the manufacturers instructions. Bca product description protein determination is one of the most common operations performed in biochemical research.
Bicinchoninic acid kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. The micro bca protein assay kit is a specialized version of the popular pierce bca protein assay for determining the protein concentration of dilute samples. Pierce bca reagents provide accurate determination of protein concentration. Pierce bca protein assay kit 23227 from thermo fisher. Bca protein assay thermo fisher scientific pdf book. Pierce bca protein assay kit thermo fisher scientific. Thermo scientific pierce bca protein assay kit protein. Smith at the pierce chemical company, now part of thermo fisher scientific, is a biochemical assay for determining the total concentration of protein in a solution 0. Pierce bca protein assay kit is a twocomponent, highprecision, detergent compatible protein assay for determination of protein concentration.
Measuring the protein concentration of samples is essential for many protein assays, such as western blotting or zymography. Provides high accuracy results in fraction of time. The thermo scientific pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure a562nm total protein concentration compared to a protein standard. Dispense from vial and place into 10ml of solution. Procedure for using pierce protease inhibitor mini tablet 1. Bca protein assay kit bicinchoninic acid protein assay kit. Determine acceptable wavelengths for measuring protein. Pool the eluted ig fractions that contain the highest absorbance. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. The purified antibodies may be used directly for sds. Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration. Prepare pierce bovine serum albumin bsa standards 2,000. Micro bca protein assay kit thermo fisher scientific. Evaluating singledomain antibodies as carriers for.
Thermo scientific pierce rapid gold bca protein assay kit. I was going to use the pierce bca protein assay kit which includes albumin standard ampules, 2 mgml, 10 x 1 ml. Pierce bca reagents provide accurate determination of protein concentration with most sample types encountered in protein research. Pierce bca protein assay kit 23225 from thermo fisher. Bca protein assay reagent bicinchoninic acid youtube. The bca protein assay kit from thermo scientific pierce product no. Bca protein assay kit from thermo scientific pierce.
Urine dip stick strips were purchased from euro diagnostic systems, india pvt. The kits include bovine serum albumin, which is used to set up a standard curve from which the. Chemical hazard bca protein assay reagent kit harmful bca protein assay reagent b toxic r. Jan 20, 2005 the micro bca protein assay kit from pierce biotechnology is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. I have a technical question regarding the bca assay. The pierce bca protein assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications.
Thermo scientific pierce bca protein assay kit remains one of the most popular protein quantitation methods worldwide. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater protein to protein uniformity. In this experiment, sarcoplasmic muscle proteins are extracted with a 0. Piercea protein assay kit bc thermo fisher scientific.
Oct 22, 2007 the bca protein assay kit from thermo scientific pierce product no. Eluted apn was dialyzed immediately in pbs and stored at. Biovisions bca protein assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents. Instructions pierce protease inhibitor mini tablets. Thermo scientific pierce microplate bca protein assay kit. Protocol the following are instructions for the microplate assay format of the pierce bca protein assay kit thermo scientific cat. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater protein to protein uniformity. Bca protein assay kit, sufficient reagents for 250 test tube or 2,500 microplate assays. Bca protein assay reagent kit, sufficient reagents for 500 test tube or 5,000.
Bicinchoninic acid protein assay kit catalog numbers bca1 and b9643 technical bulletin synonym. The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. The principle of the bicinchoninicacid bca assay is similar to the lowry procedure,1 in that both rely on the. The principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration.
Bsa bovine serum albumin is used as the standard and samples are. Wear lab coat, enclosed shoes and safety glasses when handling bca protein reagents. Thermo scientific pierce bca protein assay kit protein assay kit. An adaptation of the bca protein assay kit product no. The risk from chemical contact is low if risk controls are followed. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Use the buffer your samples are dissolved in for the diluent. Features and benefits simple, sensitive colorimetric assay for proteins two stable reagents are used for the working solution faster and easier than the lowry protein assay. If they can be stored and reused to create another standard curve, what is recommended for storage temp. Pierce bca protein assay kit, sufficient reagents for 250 testtube or 2500 microplate assays. This special adaptation of the popular pierce bca protein assay method enables accurate protein concentration measurement for samples containing dtt, 2me or tcep. Used in more labs than any other detergentcompatible protein assay, pierce bca reagents provide accurate determination of protein concentration with most sample types.
For example, a twofold dilution series from 2000 gml to. Instructions tper tissue protein extraction reagent. Thermo scientific pierce protein assay technical handbook. Bca protein assay kit is a readytouse detergent compatible western blot related total protein analysis reagent used for the quick determination of total protein concentration by measuring absorbance at 562 nm and comparing to a protein standard absorption vs.
A modulus method for bca protein assay pdf book manual. If precise quantitation of an unknown protein is required, it is advisable to select a protein. Bca is a general use kit to determine total protein concentration prior to western blot or other protein analysis. An adaptation of the thermo scientific bca protein assay kit product no. The bicinchoninic acid bca assay method will be used for this purpose. Typographical error, page formattingtable formatting, incorrectmissing document pdf, other.